Services available at the ITRC



  • Molecular identification of single larvae of Trichinella at the species or genotype level
  • Morphological identification of larvae or adults of Trichinella
  • Immunohistochemical identification of Trichinella larvae in muscle samples fixed in formalin or paraffin embedded
  • Detection of anti-Trichinella antibodies in human sera by validated ELISA and Western bot
  • Detection of anti-Trichinella antibodies in pig sera by validated ELISA and Western bot
  • Detection of anti-Trichinella antibodies in other animal species by validated ELISA and Western bot

For more information on molecular identification of Trichinella taxa read the following publications:
  • Pozio E, La Rosa G. 2010. Trichinella. In: Liu D. (Ed.), Molecular detection of Foodborne Pathogens. CRC Press, Taylor & Francis Group, Boca Raton, USA, pp. 851-863.

For more information on serology read the following publications:
  • Gómez-Morales MA, Selmi M, Ludovisi A, Amati M, Fiorentino E, Breviglieri L, Poglayen G, Pozio E. 2016. Hunting dogs as sentinel animals for monitoring infections with Trichinella spp. in wildlife. Parasit Vectors. 9:154.
  • Gómez-Morales MA, Ludovisi A, Amati M, Pozio E. 2015. Candidates for reference swine serum with anti-Trichinella antibodies. Vet Parasitol. 208:218-24.
  • Gómez-Morales MA, Ludovisi A, Amati M, Bandino E, Capelli G, Corrias F, Gelmini L, Nardi A, Sacchi C, Cherchi S, Lalle M, Pozio E. 2014. Indirect versus direct detection methods of Trichinella spp. infection in wild boar (Sus scrofa). Parasit Vectors. 7:171.
  • Gómez-Morales MA, Ludovisi A, Amati M, Blaga R, Zivojinovic M, Ribicich M, Pozio E. 2012. A distinctive Western blot pattern to recognize Trichinella infections in humans and pigs. Int J Parasitol. 42:1017-23.
  • Gómez-Morales MA, Ludovisi A, Amati M, Cherchi S, Pezzotti P, Pozio E. 2008. Validation of an enzyme-linked immunosorbent assay for diagnosis of human trichinellosis. Clin Vaccine Immunol. 15:1723-9.

Reference material available at the ITRC
  • Reference strains in vivo
  • Larvae of reference strains in ethyl alcohol
  • Crude antigen of Trichinella spiralis
  • Excretory/secretory antigen of Trichinella spiralis
  • Reference sera from Trichinella-positive humans and animals

For more information contact us by email edoardo.pozio@iss.it, giuseppe.larosa@iss.it, mariaangeles.gomezmorales@iss.it